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irradiate with ultraviolet rays中文是什么意思

  • 用紫外线照射

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  • Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants . the combined gene was cloned into plant expression vector pbi121 and then transformed . about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
    为了便于转基因棉花后代的筛选,在pap基因的3 ’端融入了绿色荧光蛋白gfp )基因,然后将融合基因克隆在植物表达载体pbi121上,再进行遗传转化,得转基因棉花种子5000余粒,将种子播种长到于叶展开时,先在黑暗中用紫外灯照射,查找表现绿色荧光的幼苗,然后再用地高辛( dig )标记的pap基因特异性探针对这些棉花进行点杂交,最后发现有8株棉花表现阳性反应,说明pap基因的确己经转到了棉花的基因组中,其棉花黄萎病的抗性鉴定正在进行之中。
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